Correlation of Influenza B Haemagglutination Inhibiton, Single-Radial Haemolysis and Pseudotype-Based Microneutralisation Assays for Immunogenicity Testing of Seasonal Vaccines

Immunogenicity and Efficacy of Different Haemophilus influenzae type b Vaccines

Haemophilus influenzae, a major cause of meningitis in young children leading to death and other neurological sequelae. The disease leaves 15 to 35% of the survivors with permanent disabilities, such as, mental retardation or deafness. Despite the availability of new and more powerful antibiotics children with Hib meningitis still suffer from high mortality or morbidity. The emergence of multir...

Pseudotype-Based Neutralization Assays for Influenza: A Systematic Analysis

The use of vaccination against the influenza virus remains the most effective method of mitigating the significant morbidity and mortality caused by this virus. Antibodies elicited by currently licensed influenza vaccines are predominantly hemagglutination-inhibition (HI)-competent antibodies that target the globular head of hemagglutinin (HA) thus inhibiting influenza virus entry into target c...

A Single Radial Haemolysis Technique for Rapid Diagnosis of Goat Pox Diseases

Quantitation and analysis of the specificity of post-immunization antibodies to influenza B viruses using single radial haemolysis.

The single radial haemolysis (SRH) technique detected anti-B/HK/8/73 HA antibody rises in 59-85% of paired sera from persons immunized with different influenza vaccines. In contrast, analysis of the same sera by the haemagglutination inhibition (HI) test indicated significant antibody rises in only 27-54% of paired sera. High levels of antibody were detected to influenza B/HK/8/73 and B/Singapo...

Chimeric influenza haemagglutinins: Generation and use in pseudotype neutralization assays

Recently chimeric influenza haemagglutinins (cHAs) have been generated as potential 'universal' vaccination antigens and as tools to identify HA stalk-directed antibodies via their use as antigens in ELISA, and virus or pseudotype-based neutralization assays. The original methods [1], [2] used for their generation require the amplification of regions of interest (head and stalk) using primers c...